Genetic variation of E6 and E7 genes of human papillomavirus type 16 from central China.

ABSTRACT:

Background

Persistent high-risk human papillomavirus (HR-HPV) infection is an important factor in the development of cervical cancer, and human papillomavirus type 16 (HPV-16) is the most common HR-HPV type worldwide. The oncogenic potential of HPV-16 is closely related to viral sequence variation.

Methods

In order to clarify the variant characteristics of HPV-16 E6 and E7 genes in central China, E6 and E7 sequences of 205 HPV-16 positive samples were amplified by polymerase chain reaction. PCR products of E6 and E7 genes were further sequenced and subjected to variation analysis, phylogenetic analysis, selective pressure analysis and B-cell epitope prediction.

Results

Twenty-six single nucleotide variants were observed in E6 sequence, including 21 non-synonymous and

SUBMITTER: Li T 

PROVIDER: S-EPMC10537582 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature