Mass cytometry profiling of human dendritic cells in blood and tissues.

ABSTRACT: The immune system comprises distinct functionally specialized cell populations, which can be characterized in depth by mass cytometry protein profiling. Unfortunately, the low-throughput nature of mass cytometry has made it challenging to analyze minor cell populations. This is the case for dendritic cells, which represent 0.2-2% of all immune cells in tissues and yet perform the critical task of initiating and modulating immune responses. Here, we provide an optimized step-by-step protocol for the characterization of well-known and emerging human dendritic cell populations in blood and tissues using mass cytometry. We provide detailed instructions for the generation of single-cell suspensions, sample enrichment, staining, acquisition and data analysis. We also include a barcoding option t

SUBMITTER: Alcantara-Hernandez M 

PROVIDER: S-EPMC10538357 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature