Anti-proliferative effect of Annona extracts on breast cancer cells.

ABSTRACT:

Backgorund

Fruits and seed extracts of Annona montana have significant cytotoxic potential in several cancer cells. This study evaluates the effect of A. montana leaves hexane extract on several signaling cascades and gene expression in metastatic breast cancer cells upon insulin-like growth factor-1 (IGF-1) stimulation.

Methods

MTT assay was performed to determine the proliferation of cancer cells. Propidium iodide staining and flow cytometry analysis of Annexin V binding was utilized to measure the progression of the cell cycle and the induction of apoptosis. Protein expression and phosphorylation were determined by western blotting analysis to examine the underlying cellular mechanism triggered upon treatment with A. montana leaves hexane extract.

Results

A.

SUBMITTER: Veisaga ML 

PROVIDER: S-EPMC10538365 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature