Loss of 12-Lipoxygenase Improves the Post-Transfusion Function of Stored Platelets.

ABSTRACT:

Background

Platelets for transfusion are stored for 5 to 7 days. Previous studies have shown that HETE levels in the storage bag negatively correlate with platelet performance in vivo, suggesting that the dysregulation of bioactive lipid mediators may contribute to the storage lesion. In the current study, we sought to understand how genetic deletion and pharmacological inhibition of 12-LOX (12-lipoxygenase) affects platelets during storage and after transfusion.

Methods

Platelets from 12-LOX^+/+ (wild-type [WT]) and 12-LOX^-/- mice were stored for 24 and 48 hours and profiled using liquid chromatography-tandem mass spectrometry-multiple reaction monitoring or transfused into thrombocytopenic hIL4R (human interleukin 4 receptor)-transgenic mice. Platelet

SUBMITTER: Larsen HJ 

PROVIDER: S-EPMC10538391 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature