High-Resolution Single-Molecule Structure Revealed by Electron Microscopy and Individual Particle Electron Tomography.

ABSTRACT: The protein is naturally dynamic and heterogeneous in solution. Protein dynamics involves both equilibrium fluctuations that regulate biological function and other non-equilibrium effects of biological motors, which convert chemical energy to mechanical energy. However, a single, unique structure of protein determined from X-ray crystal and conventional single-particle electron microscopy is insufficient to encompass the dynamic nature of proteins in solution. Structure determination of dynamic and heterogeneous protein is essentially required the determination of each individual particle of protein. Recently, Drs. Gang Ren and Lei Zhan published the first single molecule three-dimensional (3D) EM images of individual proteins ever obtained with enough clarity to determine their structure,

SUBMITTER: Zhang L 

PROVIDER: S-EPMC10538445 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature