Biomolecular condensates modulate membrane lipid packing and hydration.

ABSTRACT: Membrane wetting by biomolecular condensates recently emerged as a key phenomenon in cell biology, playing an important role in a diverse range of processes across different organisms. However, an understanding of the molecular mechanisms behind condensate formation and interaction with lipid membranes is still missing. To study this, we exploited the properties of the dyes ACDAN and LAURDAN as nano-environmental sensors in combination with phasor analysis of hyperspectral and lifetime imaging microscopy. Using glycinin as a model condensate-forming protein and giant vesicles as model membranes, we obtained vital information on the process of condensate formation and membrane wetting. Our results reveal that glycinin condensates display differences in water dynamics when changing the salin

SUBMITTER: Mangiarotti A 

PROVIDER: S-EPMC10539446 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature