Specific biomarker mining and rapid detection of Burkholderia cepacia complex by recombinase polymerase amplification.

ABSTRACT:

Objective

To mine specific proteins and their protein-coding genes as suitable molecular biomarkers for the Burkholderia cepacia Complex (BCC) bacteria detection based on mega analysis of microbial proteomic and genomic data comparisons and to develop a real-time recombinase polymerase amplification (rt-RPA) assay for rapid isothermal screening for pharmaceutical and personal care products.

Methods

We constructed an automatic screening framework based on Python to compare the microbial proteomes of 78 BCC strains and 263 non-BCC strains to identify BCC-specific protein sequences. In addition, the specific protein-coding gene and its core DNA sequence were validated in silico with a self-built genome database containing 158 thousand bacteria. The appropriate met

SUBMITTER: Fan Y 

PROVIDER: S-EPMC10539473 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature