Extended Bloch-McConnell equations for mechanistic analysis of hyperpolarized ¹³C magnetic resonance experiments on enzyme systems.

ABSTRACT: We describe an approach to formulating the kinetic master equations of the time evolution of NMR signals in reacting (bio)chemical systems. Special focus is given to studies that employ signal enhancement (hyperpolarization) methods such as dissolution dynamic nuclear polarization (dDNP) and involving nuclear spin-bearing solutes that undergo reactions mediated by enzymes and membrane transport proteins. We extend the work given in a recent presentation on this topic (Kuchel and Shishmarev, 2020) to now include enzymes with two or more substrates and various enzyme reaction mechanisms as classified by Cleland, with particular reference to non-first-order processes. Using this approach, we can address some pressing questions in the field from a theoretical standpoint. For example, why does

SUBMITTER: Eykyn TR 

PROVIDER: S-EPMC10539799 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature