ATF3 regulates SPHK1 in cardiomyocyte injury via endoplasmic reticulum stress.

ABSTRACT:

Aim

Endoplasmic reticulum (ER) stress is common in different human pathologies, including cardiac diseases. Sphingosine kinase-1 (SPHK1) represents an important player in cardiac growth and function. Nevertheless, its function in cardiomyocyte ER stress remains vague. This study sought to evaluate the mechanism through which SPHK1 might influence ER stress during myocardial infarction (MI).

Methods

MI-related GEO data sets were queried to screen differentially expressed genes. Murine HL-1 cells exposed to oxygen-glucose deprivation (OGD) and mice with MI were induced, followed by gene expression manipulation using short hairpin RNAs and overexpression vectors. The activating transcription factor 3 (ATF3) and SPHK1 expression was examined in cells and tissues. Cell counting k

SUBMITTER: Chen H 

PROVIDER: S-EPMC10540145 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature