Activity Guided Azide-methyllysine Photo-trapping for Substrate Profiling of Lysine Demethylases.

ABSTRACT: Reversible post-translational modifications (PTMs) are key to establishing protein-protein and protein-nucleic acid interactions that govern a majority of the signaling pathways in cells. Sequence-specific PTMs are catalyzed by transferases, and their removal is carried out by a class of reverse-acting enzymes termed "detransferases". Currently available chemoproteomic approaches have been valuable in characterizing substrates of transferases. However, proteome-wide cataloging of the substrates of detransferases is challenging, mostly due to the loss of the epitope, rendering immunoprecipitation and activity-based methods ineffective. Herein, we develop a general chemoproteomic strategy called crosslinking-assisted substrate identification (CASI) for systematic characterization of cellular

SUBMITTER: Kuwik J 

PROVIDER: S-EPMC10540216 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature