Alternate Strategies to Induce Dynamically Modulated Transient Transcription Machineries.

ABSTRACT: Emulating native transient transcription machineries modulating temporal gene expression by synthetic circuits is a major challenge in the area of systems chemistry. Three different methods to operate transient transcription machineries and to modulate the gated transcription processes of target RNAs are introduced. One method involves the design of a reaction module consisting of transcription templates being triggered by promoter fuel strands transcribing target RNAs and in parallel generating functional DNAzymes in the transcription templates, modulating the dissipative depletion of the active templates and the transient operation of transcription circuits. The second approach involves the application of a reaction module consisting of two transcription templates being activated by a co

SUBMITTER: Li Z 

PROVIDER: S-EPMC10540262 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature