Enhanced Vaccine Immunogenicity Enabled by Targeted Cytosolic Delivery of Tumor Antigens into Dendritic Cells.

ABSTRACT: Molecular vaccines comprising antigen peptides and inflammatory cues make up a class of therapeutics that promote immunity against cancer and pathogenic diseases but often exhibit limited efficacy. Here, we engineered an antigen peptide delivery system to enhance vaccine efficacy by targeting dendritic cells and mediating cytosolic delivery. The delivery system consists of the nontoxic anthrax protein, protective antigen (PA), and a single-chain variable fragment (scFv) that recognizes the XCR1 receptor on dendritic cells (DCs). Combining these proteins enabled selective delivery of the N-terminus of lethal factor (LF_N) into XCR1-positive cross-presenting DCs. Incorporating immunogenic epitope sequences into LF_N showed selective protein translocation in vitro and enha

SUBMITTER: Truex NL 

PROVIDER: S-EPMC10540291 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature