Mechanical stretch promotes apoptosis and impedes ciliogenesis of primary human airway basal stem cells.

ABSTRACT:

Background

Airway basal stem cells (ABSCs) have self-renewal and differentiation abilities. Although an abnormal mechanical environment related to chronic airway disease (CAD) can cause ABSC dysfunction, it remains unclear how mechanical stretch regulates the behavior and structure of ABSCs. Here, we explored the effect of mechanical stretch on primary human ABSCs.

Methods

Primary human ABSCs were isolated from healthy volunteers. A Flexcell FX-5000 Tension system was used to mimic the pathological airway mechanical stretch conditions of patients with CAD. ABSCs were stretched for 12, 24, or 48 h with 20% elongation. We first performed bulk RNA sequencing to identify the most predominantly changed genes and pathways. Next, apoptosis of stretched ABSCs was detected with Annex

SUBMITTER: Lin LQ 

PROVIDER: S-EPMC10540374 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature