Economical production of Pichia pastoris single cell protein from methanol at industrial pilot scale.

ABSTRACT:

Background

Methanol, synthesized from CO₂, is a potentially sustainable one-carbon (C1) resource for biomanufacturing. The use of methanol as a feedstock to produce single cell protein (SCP) has been investigated for decades as an alternative to alleviate the high global demand for animal-derived proteins. The methylotrophic yeast Pichia pastoris is an ideal host for methanol-based SCP synthesis due to its natural methanol assimilation ability. However, improving methanol utilization, tolerance to higher temperature, and the protein content of P. pastoris are also current challenges, which are of great significance to the economical industrial application using methanol as a feedstock for SCP production.

Results

In the present work, adaptive laboratory evolution (

SUBMITTER: Meng J 

PROVIDER: S-EPMC10540378 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature