Neuronal MHC-I complex is destabilized by amyloid-β and its implications in Alzheimer's disease.

ABSTRACT:

Backgrounds

The expression of major histocompatibility complex I (MHC-I) in neurons has recently been shown to regulate neurite outgrowth and synaptic plasticity. However, its contribution to neurodegenerative diseases such as Alzheimer's disease (AD) remains largely unknown.

Methods

In this study, we investigated the relationship between impaired MHC-I-β2M complex and AD in vitro and human AD samples. Interaction between protein was identified by liquid chromatography-tandem mass spectrometry and confirmed by immunoprecipitation. Single-chain trimer of MHC-I-β2M was generated to study the effect of stabilization of MHC-I-β2M complex on NCAM1 signaling.

Results

MHC-I is destabilized in the brains of AD patients and neuronal cells treated with oligomeric β-amyloid (Aβ)

SUBMITTER: Kim MS 

PROVIDER: S-EPMC10540404 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature