Changes in m⁶A RNA methylation are associated with male sterility in wolfberry.

ABSTRACT:

Background

N⁶-methyladenosine (m⁶A) modification is the most abundant type of RNA modification in eukaryotic cells, playing pivotal roles in multiple plant growth and development processes. Yet the potential role of m⁶A in conferring the trait of male sterility in plants remains unknown.

Results

In this study, we performed RNA-sequencing (RNA-Seq) and m⁶A-sequencing (m⁶A-Seq) of RNAs obtained from the anther tissue of two wolfberry lines: 'Ningqi No.1' (LB1) and its natural male sterile mutant 'Ningqi No.5' (LB5). Based on the newly assembled transcriptome, we established transcriptome-wide m⁶A maps for LB1 and LB5 at the single nucleus pollen stage. We found that the gene XLOC_021201, a homolog of m⁶

SUBMITTER: Zhao J 

PROVIDER: S-EPMC10540408 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature