Lung immune signatures define two groups of end-stage IPF patients.

ABSTRACT:

Background

The role of the immune system in the pathobiology of Idiopathic Pulmonary Fibrosis (IPF) is controversial.

Methods

To investigate it, we calculated immune signatures with Gene Set Variation Analysis (GSVA) and applied them to the lung transcriptome followed by unbiased cluster analysis of GSVA immune-enrichment scores, in 109 IPF patients from the Lung Tissue Research Consortium (LTRC). Results were validated experimentally using cell-based methods (flow cytometry) in lung tissue of IPF patients from the University of Pittsburgh (n = 26). Finally, differential gene expression and hypergeometric test were used to explore non-immune differences between clusters.

Results

We identified two clusters (C#1 and C#2) of IPF patients of similar size in the LTRC datas

SUBMITTER: Cruz T 

PROVIDER: S-EPMC10540496 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature