BoGH13A_Sus from Bacteroides ovatus represents a novel α-amylase used for  Bacteroides starch breakdown in the human gut.

ABSTRACT: Members of the Bacteroidetes phylum in the human colon deploy an extensive number of proteins to capture and degrade polysaccharides. Operons devoted to glycan breakdown and uptake are termed polysaccharide utilization loci or PUL. The starch utilization system (Sus) is one such PUL and was initially described in Bacteroides thetaiotaomicron (Bt). BtSus is highly conserved across many species, except for its extracellular α-amylase, SusG. In this work, we show that the Bacteroides ovatus (Bo) extracellular α-amylase, BoGH13A_Sus, is distinguished from SusG in its evolutionary origin and its domain architecture and by being the most prevalent form in Bacteroidetes Sus. BoGH13A_Sus is the founding member of both a novel subfamily in the glycoside hydrolase family 13, GH13

SUBMITTER: Brown HA 

PROVIDER: S-EPMC10540511 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature