A phospho-dawn of protein modification anticipates light onset in the picoeukaryote Ostreococcus tauri.

ABSTRACT: Diel regulation of protein levels and protein modification had been less studied than transcript rhythms. Here, we compare transcriptome data under light-dark cycles with partial proteome and phosphoproteome data, assayed using shotgun MS, from the alga Ostreococcus tauri, the smallest free-living eukaryote. A total of 10% of quantified proteins but two-thirds of phosphoproteins were rhythmic. Mathematical modelling showed that light-stimulated protein synthesis can account for the observed clustering of protein peaks in the daytime. Prompted by night-peaking and apparently dark-stable proteins, we also tested cultures under prolonged darkness, where the proteome changed less than under the diel cycle. Among the dark-stable proteins were prasinophyte-specific sequences that were also repor

SUBMITTER: Noordally ZB 

PROVIDER: S-EPMC10540734 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature