The influence of intervertebral disc overloading on nociceptor calcium flickering.

ABSTRACT:

Introduction

Mechanical overloading can trigger a degenerative-like cascade in an organ culture of intervertebral disc (IVD). Whether the overloaded IVD can influence the activation of nociceptors (i.e., the damage sensing neurons) remains unknown. The study aims to investigate the influence of overloaded IVD conditioned medium (CM) on the activation of nociceptors.

Methods

In the static loading regime, force-controlled loading of 0.2 MPa for 20 h/day representing "long-term sitting and standing" was compared with a displacement-controlled loading maintaining original IVD height. In the dynamic loading regime, high-frequency-intensity loading representing degenerative "wear and tear" was compared with a lower-frequency-intensity loading. CM of differently loaded IVDs were co

SUBMITTER: Gewiess J 

PROVIDER: S-EPMC10540821 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature