Recommendations for intervertebral disc notochordal cell investigation: From isolation to characterization.

ABSTRACT:

Background

Lineage-tracing experiments have established that the central region of the mature intervertebral disc, the nucleus pulposus (NP), develops from the embryonic structure called "the notochord". However, changes in the cells derived from the notochord which form the NP (i.e., notochordal cells [NCs]), in terms of their phenotype and functional identity from early developmental stages to skeletal maturation are less understood. These key issues require further investigation to better comprehend the role of NCs in homeostasis and degeneration as well as their potential for regeneration. Progress in utilizing NCs is currently hampered due to poor consistency and lack of consensus methodology for in vitro NC extraction, manipulation, and characterization.

Methods

Here,

SUBMITTER: Williams RJ 

PROVIDER: S-EPMC10540834 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature