USP48/USP31 Is a Nuclear Deubiquitinase that Potently Regulates Synapse Remodeling.

ABSTRACT: Deubiquitinases present locally at synapses regulate synaptic development, function, and plasticity. It remains largely unknown, however, whether deubiquitinases localized outside of the synapse control synapse remodeling. Here we identify ubiquitin specific protease 48 (USP48; formerly USP31) as a nuclear deubiquitinase mediating robust synapse removal. USP48 is expressed primarily during the first postnatal week in the rodent brain and is virtually restricted to nuclei, mediated by a conserved, 13-amino acid nuclear localization signal. When exogenously expressed, USP48, in a deubiquitinase and nuclear localization-dependent manner, induces striking filopodia elaboration, marked spine loss, and significantly reduced synaptic protein clustering in vitro, and erases ~70% of functional syna

SUBMITTER: Ma Q 

PROVIDER: S-EPMC10541093 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature