GammaGateR: semi-automated marker gating for single-cell multiplexed imaging.

ABSTRACT:

Motivation

Multiplexed immunofluorescence (mIF) is an emerging assay for multichannel protein imaging that can decipher cell-level spatial features in tissues. However, existing automated cell phenotyping methods, such as clustering, face challenges in achieving consistency across experiments and often require subjective evaluation. As a result, mIF analyses often revert to marker gating based on manual thresholding of raw imaging data.

Results

To address the need for an evaluable semi-automated algorithm, we developed GammaGateR, an R package for interactive marker gating designed specifically for segmented cell-level data from mIF images. Based on a novel closed-form gamma mixture model, GammaGateR provides estimates of marker-positive cell proportions and soft clustering

SUBMITTER: Xiong J 

PROVIDER: S-EPMC10541135 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature