G-actin diffusion is insufficient to achieve F-actin assembly in fast-treadmilling protrusions.

ABSTRACT: To generate forces that drive migration of a eukaryotic cell, arrays of actin filaments (F-actin) are assembled at the cell's leading membrane edge. To maintain cell propulsion and respond to dynamic external cues, actin filaments must be disassembled to regenerate the actin monomers (G-actin), and transport of G-actin from sites of disassembly back to the leading edge completes the treadmilling cycle and limits the flux of F-actin assembly. Whether or not molecular diffusion is sufficient for G-actin transport has been a long-standing topic of debate, in part because the dynamic nature of cell motility and migration hinders the estimation of transport parameters. In this work, we applied an experimental system in which cells adopt an approximately constant and symmetrical shape; they cann

SUBMITTER: Appalabhotla R 

PROVIDER: S-EPMC10541494 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature