Beyond analytic solution: Analysis of FRAP experiments by spatial simulation of the forward problem.

ABSTRACT: Fluorescence redistribution after photobleaching is a commonly used method to understand the dynamic behavior of molecules within cells. Analytic solutions have been developed for specific, well-defined models of dynamic behavior in idealized geometries, but these solutions are inaccurate in complex geometries or when complex binding and diffusion behaviors exist. We demonstrate the use of numerical reaction-diffusion simulations using the Virtual Cell software platform to model fluorescence redistribution after photobleaching experiments. Multiple simulations employing parameter scans and varying bleaching locations and sizes can help to bracket diffusion coefficients and kinetic rate constants in complex image-based geometries. This approach is applied to problems in membrane surface dif

SUBMITTER: Cowan AE 

PROVIDER: S-EPMC10541496 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature