Gut-targeted nanoparticles deliver specifically targeted antimicrobial peptides against Clostridium perfringens infections.

ABSTRACT: Specifically targeted antimicrobial peptides (STAMPs) are novel alternatives to antibiotics, whereas the development of STAMPs for colonic infections is hindered by limited de novo design efficiency and colonic bioavailability. In this study, we report an efficient de novo STAMP design strategy that combines a traversal design, machine learning model, and phage display technology to identify STAMPs against Clostridium perfringens. STAMPs could physically damage C. perfringens, eliminate biofilms, and self-assemble into nanoparticles to entrap pathogens. Further, a gut-targeted engineering particle vaccine (EPV) was used for STAMPs delivery. In vivo studies showed that both STAMP and EPV@STAMP effectively limited C. perfringens infections and then reduced inflammatory r

SUBMITTER: Xu B 

PROVIDER: S-EPMC10541502 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature