Sevoflurane anaesthesia induces cognitive impairment in young mice through sequential tau phosphorylation.

ABSTRACT:

Background

The volatile anaesthetic sevoflurane induces time (single or multiple exposures)-dependent effects on tau phosphorylation and cognitive function in young mice. The underlying mechanism for this remains largely undetermined.

Methods

Mice received 3% sevoflurane for 0.5 h or 2 h daily for 3 days on postnatal day (P) 6, 9, and 12. Another group of mice received 3% sevoflurane for 0.5 h or 1.5 h (3 × 0.5) on P6. We investigated effects of sevoflurane anaesthesia on tau phosphorylation on P6 or P12 mice, on cognitive function from P31 to P37, and on protein interactions, using in vivo studies, in vitro phosphorylation assays, and nanobeam single-molecule level interactions in vitro.

Results

An initial sevoflurane exposure induced CaMKIIα phosphorylation (132 [11

SUBMITTER: Liang F 

PROVIDER: S-EPMC10541551 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature