Novel endolysin LysMP for control of Limosilactobacillus fermentum contamination in small-scale corn mash fermentation.

ABSTRACT:

Background

Traditional bioethanol fermentation industries are not operated under strict sterile conditions and are prone to microbial contamination. Lactic acid bacteria (LAB) are often pervasive in fermentation tanks, competing for nutrients and producing inhibitory acids that have a negative impact on ethanol-producing yeast, resulting in decreased yields and stuck fermentations. Antibiotics are frequently used to combat contamination, but antibiotic stewardship has resulted in a shift to alternative antimicrobials.

Results

We demonstrate that endolysin LysMP, a bacteriophage-encoded peptidoglycan hydrolase, is an effective method for controlling growth of LAB. The LysMP gene was synthesized based on the prophage sequence in the genome of Limosilactobacillus fermentum KGL7

SUBMITTER: Patel MH 

PROVIDER: S-EPMC10541714 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature