Multi-target genome editing reduces polyphenol oxidase activity in wheat (Triticum aestivum L.) grains.

ABSTRACT:

Introduction

Polyphenol oxidases (PPO) are dual activity metalloenzymes that catalyse the production of quinones. In plants, PPO activity may contribute to biotic stress resistance and secondary metabolism but is undesirable for food producers because it causes the discolouration and changes in flavour profiles of products during post-harvest processing. In wheat (Triticum aestivum L.), PPO released from the aleurone layer of the grain during milling results in the discolouration of flour, dough, and end-use products, reducing their value. Loss-of-function mutations in the PPO1 and PPO2 paralogous genes on homoeologous group 2 chromosomes confer reduced PPO activity in the wheat grain. However, limited natural variation and the proximity of these genes complicates the select

SUBMITTER: Wold-McGimsey F 

PROVIDER: S-EPMC10541959 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature