Massively parallel in vivo Perturb-seq reveals cell type-specific transcriptional networks in cortical development.

ABSTRACT: Systematic analysis of gene function across diverse cell types in vivo is hindered by two challenges: obtaining sufficient cells from live tissues and accurately identifying each cell's perturbation in high-throughput single-cell assays. Leveraging AAV's versatile cell type tropism and high labeling capacity, we expanded the resolution and scale of in vivo CRISPR screens: allowing phenotypic analysis at single-cell resolution across a multitude of cell types in the embryonic brain, adult brain, and peripheral nervous system. We undertook extensive tests of 86 AAV serotypes, combined with a transposon system, to substantially amplify labeling and accelerate in vivo gene delivery from weeks to days. Using this platform, we performed an in utero genetic screen as p

SUBMITTER: Zheng X 

PROVIDER: S-EPMC10542124 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature