A tool to automatically design multiplex PCR primer pairs for specific targets using diverse templates.

ABSTRACT: Multiplex PCR is an increasingly popular method for identifying species, investigating environmental diversity, and conducting phylogenetic analysis. The complexity and increasing availability of diverse templates necessitate a highly automated approach to design degenerate primer pairs for specific targets with multiple sequences. Existing tools for degenerate primer design suffer from poor maintenance, semi-automation, low adaptability, and low tolerance for gaps. We developed PMPrimer, a Python-based tool for automated design and evaluation of multiplex PCR primer pairs for specific targets using diverse templates. PMPrimer automatically designs optimal multiplex PCR primer pairs using a statistical-based template filter; performs multiple sequence alignment, conserved region identifica

SUBMITTER: Yang L 

PROVIDER: S-EPMC10542359 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature