Innate immune dysregulation in multisystem inflammatory syndrome in children (MIS-C).

ABSTRACT: MIS-C is a systemic inflammation disorder with poorly characterised immunopathological mechanisms. We compared changes in the systemic immune response in children with MIS-C (n = 12, 5-13 years) to healthy controls (n = 14, 5-15 years). Analysis was done in whole blood treated with LPS. Expression of CD11b and Toll-like receptor-4 (TLR4) in neutrophils and monocytes were analysed by flow cytometry. Serum cytokines (IL-1β, IL-2, IL-6, IL-8, IL-10, IL-Ira, TNF-α, TNF-β, IFN-Υ, VEGF, EPO and GM-CSF) and mRNA levels of inflammasome molecules (NLRP3, ASC and IL-1β) were evaluated. Subpopulations of lymphocytes (CD3⁺, CD19⁺, CD56⁺, CD4⁺, CD8⁺, TCR Vδ1⁺, TCR Vδ2⁺) were assessed at basal levels. Absolute counts of neutrop

SUBMITTER: Isaza-Correa J 

PROVIDER: S-EPMC10542373 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature