Characterization of integration sites and transfer DNA structures in Agrobacterium-mediated transgenic events of maize inbred B104.

ABSTRACT: In maize, the community-standard transformant line B104 is a useful model for dissecting features of transfer DNA (T-DNA) integration due to its compatibility with Agrobacterium-mediated transformation and the availability of its genome sequence. Knowledge of transgene integration sites permits the analysis of the genomic environment that governs the strength of gene expression and phenotypic effects due to the disruption of an endogenous gene or regulatory element. In this study, we optimized a fusion primer and nested integrated PCR (FPNI-PCR) technique for T-DNA detection in maize to characterize the integration sites of 89 T-DNA insertions in 81 transformant lines. T-DNA insertions preferentially occurred in gene-rich regions and regions distant from centromeres. Integration junctions

SUBMITTER: Neelakandan AK 

PROVIDER: S-EPMC10542558 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature