Probing the physiological role of the plastid outer-envelope membrane using the oemiR plasmid collection.

ABSTRACT: Plastids are the site of complex biochemical pathways, most prominently photosynthesis. The organelle evolved through endosymbiosis with a cyanobacterium, which is exemplified by the outer envelope membrane that harbors more than 40 proteins in Arabidopsis. Their evolutionary conservation indicates high significance for plant cell function. While a few proteins are well-studied as part of the protein translocon complex the majority of outer envelope protein functions is unclear. Gaining a deeper functional understanding has been complicated by the lack of observable loss-of-function mutant phenotypes, which is often rooted in functional genetic redundancy. Therefore, we designed outer envelope-specific artificial micro RNAs (oemiRs) capable of downregulating transcripts from several loci s

SUBMITTER: Schwenkert S 

PROVIDER: S-EPMC10542568 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature