FREQ-Seq2: a method for precise high-throughput combinatorial quantification of allele frequencies.

ABSTRACT: The accurate determination of allele frequencies is crucially important across a wide range of problems in genetics, such as developing population genetic models, making inferences from genome-wide association studies, determining genetic risk for diseases, as well as other scientific and medical applications. Furthermore, understanding how allele frequencies change over time in populations is central to ascertaining their evolutionary dynamics. We present a precise, efficient, and economical method (FREQ-Seq2) for quantifying the relative frequencies of different alleles at loci of interest in mixed population samples. Through the creative use of paired barcode sequences, we exponentially increased the throughput of the original FREQ-Seq method from 48 to 2,304 samples. FREQ-Seq2 can be t

SUBMITTER: Zhao R 

PROVIDER: S-EPMC10542570 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature