Decoding stoichiometric protein synthesis in E. coli through translation rate parameters.

ABSTRACT: E. coli is one of the most widely used organisms for understanding the principles of cellular and molecular genetics. However, we are yet to understand the origin of several experimental observations related to the regulation of gene expression in E. coli. One of the prominent examples in this context is the proportional synthesis in multiprotein complexes where all of their obligate subunits are produced in proportion to their stoichiometry. In this work, by combining the next-generation sequencing data with the stochastic simulations of protein synthesis, we explain the origin of proportional protein synthesis in multicomponent complexes. We find that the estimated initiation rates for the translation of all subunits in those complexes are proportional to their stoichiometr

SUBMITTER: Irshad IU 

PROVIDER: S-EPMC10542608 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature