Spatially multicellular variability of intervertebral disc degeneration by comparative single-cell analysis.

ABSTRACT: Previous studies have revealed cellular heterogeneity in intervertebral discs (IVDs). However, the cellular and molecular alteration patterns of cell populations during degenerative progression remain to be fully elucidated. To illustrate the cellular and molecular alteration of cell populations in intervertebral disc degeneration (IDD), we perform single cell RNA sequencing on cells from four anatomic sites of healthy and degenerative goat IVDs. EGLN3⁺ StressCs, TGFBR3⁺ HomCs and GPRC5A⁺ RegCs exhibit the characteristics associated with resistance to stress, maintaining homeostasis and repairing, respectively. The frequencies and signatures of these cell clusters fluctuate with IDD. Notably, the chondrogenic differentiation programme of PROCR⁺ p

SUBMITTER: Lin P 

PROVIDER: S-EPMC10542621 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature