Membrane Remodeling of Human-Engineered Cardiac Tissue by Chronic Electric Stimulation.

ABSTRACT: Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) show immature features, but these are improved by integration into 3D cardiac constructs. In addition, it has been demonstrated that physical manipulations such as electrical stimulation (ES) are highly effective in improving the maturation of human-engineered cardiac tissue (hECT) derived from hiPSC-CMs. Here, we continuously applied an ES in capacitive coupling configuration, which is below the pacing threshold, to millimeter-sized hECTs for 1-2 weeks. Meanwhile, the structural and functional developments of the hECTs were monitored and measured using an array of assays. Of particular note, a nanoscale imaging technique, scanning ion conductance microscopy (SICM), has been used to directly image membrane remodeling of

SUBMITTER: Sesena-Rubfiaro A 

PROVIDER: S-EPMC10542861 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature