Deciphering m6A dynamics at a single-base level during planarian anterior-posterior axis specification.

ABSTRACT:

Background

The establishment of the anterior-posterior (A-P) axis is a crucial step during tissue repair and regeneration. Despite the association reported recently of N⁶-methyladenosine (m6A) with regeneration, the mechanism underlying the regulation of m6A in A-P axis specification during regeneration remains unknown. Herein, we deciphered the m6A landscape at a single-base resolution at multiple time points during A-P axis regeneration and constructed the de novo transcriptome assembly of the Dugesia japonica planarian.

Results

Immunofluorescence staining and comparative analysis revealed that m6A is widespread across the planarian and dynamically regulated during regeneration along the A-P axis, exhibiting a strong spatiotemporal fe

SUBMITTER: Chen L 

PROVIDER: S-EPMC10542940 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature