Metabolomic signatures distinguish extracellular vesicles from osteoclasts and odontoclasts.

ABSTRACT:

Aims

Pathological dental root resorption and alveolar bone loss are often detected only after irreversible damage. Biomarkers in the gingival crevicular fluid or saliva could provide a means for early detection; however, such biomarkers have proven elusive. We hypothesize that a multiomic approach might yield reliable diagnostic signatures for root resorption and alveolar bone loss. Previously, we showed that extracellular vesicles (EVs) from osteoclasts and odontoclasts differ in their protein composition. In this study, we investigated the metabolome of EVs from osteoclasts, odontoclasts and clasts (non-resorbing clastic cells).

Materials and methods

Mouse haematopoietic precursors were cultured on dentine, bone or plastic, in the presence of recombinant RANKL and CSF-1 to

SUBMITTER: Rody WJ 

PROVIDER: S-EPMC10542960 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature