Candidate effectors for leaf rust resistance gene Lr28 identified through transcriptome and in-silico analysis.

ABSTRACT: Puccinia spp. causing rust diseases in wheat and other cereals secrete several specialized effector proteins into host cells. Characterization of these proteins and their interaction with host's R proteins could greatly help to limit crop losses due to diseases. Prediction of effector proteins by combining the transcriptome analysis and multiple in-silico approaches is gaining importance in revealing the pathogenic mechanism. The present study involved identification of 13 Puccinia triticina (Pt) coding sequences (CDSs), through transcriptome analysis, that were differentially expressed during wheat-leaf rust interaction; and prediction of their effector like features using different in-silico tools. NCBI-BLAST and pathogen-host interaction BLAST (PHI-BLA

SUBMITTER: Prasad P 

PROVIDER: S-EPMC10543267 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature