Role of YAP in hematopoietic differentiation and erythroid lineage specification of human-induced pluripotent stem cells.

ABSTRACT:

Background

In vitro production of hematopoietic stem/progenitor cells (HSPCs) from human-induced pluripotent stem cells (hiPSCs) provides opportunities for fundamental research, disease modeling, and large-scale production of HLA-matched HSPCs for therapeutic applications. However, a comprehensive understanding of the signaling mechanisms that regulate human hematopoiesis is needed to develop a more effective procedure for deriving HSPCs from hiPSCs.

Methods

In this study, we investigate the role of YAP during the hematopoietic differentiation of hiPSCs to HSPCs and erythrocytes using the isogenic YAP-overexpressing (YAP-S5A) and YAP-depleting (YAP-KD) hiPSCs to eliminate the effects of a genetic background variation.

Results

Although YAP is dispensable for maintainin

SUBMITTER: Laowtammathron C 

PROVIDER: S-EPMC10543272 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature