Anticodon sequence determines the impact of mistranslating tRNA^Ala variants.

ABSTRACT: Transfer RNAs (tRNAs) maintain translation fidelity through accurate charging by their cognate aminoacyl-tRNA synthetase and codon:anticodon base pairing with the mRNA at the ribosome. Mistranslation occurs when an amino acid not specified by the genetic message is incorporated into proteins and has applications in biotechnology, therapeutics and is relevant to disease. Since the alanyl-tRNA synthetase uniquely recognizes a G3:U70 base pair in tRNA^Ala and the anticodon plays no role in charging, tRNA^Ala variants with anticodon mutations have the potential to mis-incorporate alanine. Here, we characterize the impact of the 60 non-alanine tRNA^Ala anticodon variants on the growth of Saccharomyces cerevisiae. Overall, 36 tRNA^Ala anticodon vari

SUBMITTER: Cozma E 

PROVIDER: S-EPMC10543346 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature