Longitudinal assessment of mitochondrial dysfunction in acute traumatic brain injury using hyperpolarized [1-¹³ C]pyruvate.

ABSTRACT:

Purpose

[¹³ C]Bicarbonate formation from hyperpolarized [1-¹³ C]pyruvate via pyruvate dehydrogenase, a key regulatory enzyme, represents the cerebral oxidation of pyruvate and the integrity of mitochondrial function. The present study is to characterize the chronology of cerebral mitochondrial metabolism during secondary injury associated with acute traumatic brain injury (TBI) by longitudinally monitoring [¹³ C]bicarbonate production from hyperpolarized [1-¹³ C]pyruvate in rodents.

Methods

Male Wistar rats were randomly assigned to undergo a controlled-cortical impact (CCI, n = 31) or sham surgery (n = 22). Seventeen of the CCI and 9 of the sham rats longitudinally underwent a ¹ H/¹³ C-integrated MR protocol

SUBMITTER: Hackett EP 

PROVIDER: S-EPMC10543630 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature