A versatile approach to high-density microcrystals in lipidic cubic phase for room-temperature serial crystallography.

ABSTRACT: Serial crystallography has emerged as an important tool for structural studies of integral membrane proteins. The ability to collect data from micrometre-sized weakly diffracting crystals at room temperature with minimal radiation damage has opened many new opportunities in time-resolved studies and drug discovery. However, the production of integral membrane protein microcrystals in lipidic cubic phase at the desired crystal density and quantity is challenging. This paper introduces VIALS (versatile approach to high-density microcrystals in lipidic cubic phase for serial crystallography), a simple, fast and efficient method for preparing hundreds of microlitres of high-density microcrystals suitable for serial X-ray diffraction experiments at both synchrotron and free-electron laser sourc

SUBMITTER: Birch J 

PROVIDER: S-EPMC10543674 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature