MicroRNAs associated with AGL6 and IAA9 function in tomato fruit set.

ABSTRACT:

Objective

Fruit set is triggered after ovule fertilization, as a consequence of the downregulation of ovary growth repressors, such as the tomato transcription factors Auxin/indole-3-acetic acid 9 (IAA9) and Agamous-like 6 (AGL6). In a recent work, we developed a method to silence IAA9 and AGL6 in tomato ovaries using exogenous dsRNAs. We also produced small RNA libraries from IAA9- and AGL6-silenced ovaries to confirm the presence of siRNAs, derived from exogenous dsRNA, targeting IAA9 and AGL6. The objective of this work is to exploit these sRNA libraries to identify miRNAs differentially expressed in IAA9- and AGL6-silenced ovaries as compared with unpollinated control ovaries.

Results

We identified by RNA sequencing 125 and 104 known and 509 and 516 novel miRNAs from rea

SUBMITTER: Molesini B 

PROVIDER: S-EPMC10544166 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature