Mettl1-mediated internal m⁷G methylation of Sptbn2 mRNA elicits neurogenesis and anti-alzheimer's disease.

ABSTRACT:

Background

N⁷-methylguanosine (m⁷G) is one of the most conserved modifications in nucleosides impacting mRNA export, splicing, and translation. However, the precise function and molecular mechanism of internal mRNA m⁷G methylation in adult hippocampal neurogenesis and neurogenesis-related Alzheimer's disease (AD) remain unknown.

Results

We profiled the dynamic Mettl1/Wdr4 expressions and m⁷G modification during neuronal differentiation of neural stem cells (NSCs) in vitro and in vivo. Adult hippocampal neurogenesis and its molecular mechanisms were examined by morphology, biochemical methods and biological sequencing. The translation efficiency of mRNA was detected by polysome profiling. The stability of Sptbn2 mRNA was constructed

SUBMITTER: Li Q 

PROVIDER: S-EPMC10544167 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature