Screening and identifying of biomarkers in early colorectal cancer and adenoma based on genome-wide methylation profiles.

ABSTRACT:

Background

Colorectal cancer is one of the most common malignant tumors worldwide with high morbidity and mortality. This study aimed to identify different methylation sites as new methylation markers in CRC and colorectal adenoma through tissue detection.

Methods

DNA extraction and bisulfite modification as well as Infinium 450K methylation microarray detection were performed in 46 samples of sporadic colorectal cancer tissue, nine samples of colorectal adenoma, and 20 normal samples, and bioinformatic analysis was conducted involving genes enrichments of GO and KEGG. Pyrosequencing methylation detection was further performed in 68 sporadic colorectal cancer tissues, 31 samples of colorectal adenoma, and 49 normal colorectal mucosae adjacent to carcinoma to investigate the

SUBMITTER: He C 

PROVIDER: S-EPMC10544418 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature