Transcriptomic profiling reveals distinct subsets of immune checkpoint inhibitor induced myositis.

ABSTRACT:

Objectives

Inflammatory myopathy or myositis is a heterogeneous family of immune-mediated diseases including dermatomyositis (DM), antisynthetase syndrome (AS), immune-mediated necrotising myopathy (IMNM) and inclusion body myositis (IBM). Immune checkpoint inhibitors (ICIs) can also cause myositis (ICI-myositis). This study was designed to define gene expression patterns in muscle biopsies from patients with ICI-myositis.

Methods

Bulk RNA sequencing was performed on 200 muscle biopsies (35 ICI-myositis, 44 DM, 18 AS, 54 IMNM, 16 IBM and 33 normal muscle biopsies) and single nuclei RNA sequencing was performed on 22 muscle biopsies (seven ICI-myositis, four DM, three AS, six IMNM and two IBM).

Results

Unsupervised clustering defined three distinct transcriptomic subse

SUBMITTER: Pinal-Fernandez I 

PROVIDER: S-EPMC10545139 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature