VE-CADHERIN is expressed transiently in early ISL1⁺ cardiovascular progenitor cells and facilitates cardiac differentiation.

ABSTRACT: Adherens junctions (AJs) provide adhesive properties through cadherins and associated cytoplasmic catenins and participate in morphogenetic processes. We examined AJs formed between ISL1⁺ cardiovascular progenitor cells during differentiation of embryonic stem cells (ESCs) in vitro and in mouse embryogenesis in vivo. We found that, in addition to N-CADHERIN, a percentage of ISL1⁺ cells transiently formed vascular endothelial (VE)-CADHERIN-mediated AJs during in vitro differentiation on days 4 and 5, and the same pattern was observed in vivo. Fluorescence-activated cell sorting (FACS) analysis extended morphological data showing that VE-CADHERIN⁺/ISL1⁺ cells constitute a significant percentage of cardiac progenitors on days 4 and 5. The VE-CADHERI

SUBMITTER: Maltabe VA 

PROVIDER: S-EPMC10545488 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature